Taq-DNA-Polymerase.net                                Provided by ShineGene
    English Chinese     Taq DNA Polymerase Taq DNA Polymerase    

PowerQ® Taq DNA Polymerase for qPCR

Catalog #
Pack size

1.Storage condition: The undiluted enzyme solution is stable when stored at -20℃.

2.Storage and dilution buffer: 20mM Tris-HCl; 1mM dithiothreitol; 0.1mM EDTA; 0.1M KCl; Nonidet P40, 0.5%(v/v); Tween 20, 0.5%(v/v); glycerol, 50%(v/v); pH 8.0 (4℃).


4.Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10mmols of dNTP into an acid-insoluble material in 30 minutes at 74. The reaction conditions are: 50mM Tris-HCl, (pH 9.0 at 25°C), 50mM NaCl, 10Mm MgCl2, 200uM dATP, dCTP, dGTP and radiolabelled dTTP, and 12.5ug activated calf thymus DNA in a 50ul reaction.

5.10XReaction buffer(without MgCl2): 100mM Tris-HCl; 500mM KCl; pH 9.0 (20). Buffer is optimized for use with 0.2mM for each of dNTPs. A tube of 25mM MgCl2 is supplied separately.

6.Note: It is recommended to add dNTPs to this incubation mixture shortly before use. This is to prevent decomposition of the deoxynucleoside thiphosphate that occurs during Prolonged storage at the alkaline pH values required for optimal enzyme activity.

7.Quality control

Each lot of Taq DNA Polymerase is tested for activity in PCR and efficient incorporation of digoxigenin-11-dUTP, and in DNA sequencing of M13mp18ssDNA. A minimum of 250 bases must be clearly legible in the sequencing gel. Each lot of Taq DNA ploymerase is tested for contaminating activities as stated below.

8.Test buffer: 10mM Tris-HCl; 1.5mM MgCl2; 50mM KCl; pH 9.0 (20). 0.1mg/ml gelatin.

9.Absence of endonucleases: 1ul lambda DNA is incubated with Taq Dna polymerase in 50ul test buffer with a paraffin oil overlay at 65 for 16 hours. The amount of enzyme showing no degradation of the lambda DNA is ststed under "Endol".
Taq DNA Polymerase manual
If you want to get bulk order price,please contact us.E-mail:master@shinegene.org.cn or shinegene@vip.163.com

Related Link
Peptide Synthesis
Make Antibody
Lab Consumables
Gene Synthesis

Quick link:Pfu DNA Polymerase 2X Taq PCR Master Mix T4 DNA Ligase Uracil DNA Glycosylase (UNG) ShineProbe® Real Time qPCR MasterMix ShineSybr® Real Time qPCR MasterMix Kits Reverse Transcriptase 100bp DNA Marker dNTP

Copyright 2003-2008 Shanghai ShineGene Molecular Biotech,Inc. All Rights Reserved.
Floor 2,Building A,328#, Wuhe Road,Minhang District,Shanghai,201109,China
Tel:0086-21-54460832    Fax:0086-21-54460831-13    
E-mail:master@shinegene.org.cn or shinegene@vip.163.com


友情链接1:老虎机 金沙娱乐场 立博 立博博彩 六合彩 六合彩开奖 六合彩开奖结果 龙虎斗 解开美女胸衣 乱伦小说 轮盘 m88 m88.com 明升 明升国际 明升m88 明升m88.com 明升体育 明升娱乐 牛牛赌博 二十一点 轮盘 德州扑克 骰宝 博彩评级 博狗备用网址 体育博彩 彩票博彩 立博博彩 二十一点 轮盘 德州扑克 骰宝 博彩评级 博狗备用网址 体育博彩 彩票博彩 网上赌球 赌博网 澳门赌博网站 澳门赌博攻略 外围赌博网站 百家乐官网 网络百家乐 澳门百家乐网站 bet365体育在线投注 bet365开户 bet365网上赌场 bet365备用网站 体育在线 皇冠开户 皇冠网址 皇冠足球投注 hg1088.com 全讯网新2 永利高网址 永利高网上赌场 澳门外围赌博网站 太阳城官网 太阳城现金网 立博博彩