unit is defined as the amount of enzyme required to
catalyze the incorporation of 10mmols of dNTP into an
acid-insoluble material in 30 minutes at 74.
The reaction conditions are: 50mM Tris-HCl, (pH 9.0 at
25°C), 50mM NaCl, 10Mm MgCl2,
200uM dATP, dCTP, dGTP and radiolabelled dTTP, and
12.5ug activated calf thymus DNA in a 50ul reaction.
buffer(without MgCl2): 100mM
Tris-HCl; 500mM KCl; pH 9.0 (20℃). Buffer is optimized
for use with 0.2mM for each of dNTPs. A tube of 25mM
is supplied separately.
is recommended to add dNTPs to this incubation mixture
shortly before use. This is to prevent decomposition of
the deoxynucleoside thiphosphate that occurs during
Prolonged storage at the alkaline pH values required for
optimal enzyme activity.
lot of Taq DNA Polymerase is tested for activity in PCR
and efficient incorporation of digoxigenin-11-dUTP, and
in DNA sequencing of M13mp18ssDNA. A minimum of 250
bases must be clearly legible in the sequencing gel.
Each lot of Taq DNA ploymerase is tested for
contaminating activities as stated below.
of endonucleases: 1ul
lambda DNA is incubated with Taq Dna polymerase in 50ul
test buffer with a paraffin oil overlay at 65℃for
16 hours. The amount of enzyme showing no degradation of
the lambda DNA is ststed under "Endol". Taq
DNA Polymerase manual If you want to get bulk order
price,please contact us.E-mail:email@example.com